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Image Search Results
Journal: bioRxiv
Article Title: Chromosome end protection by RAP1-mediated inhibition of DNA-PK
doi: 10.1101/2024.12.28.630583
Figure Lengend Snippet: a. Outline of DNaseI footprinting experiment. 32 P-labelled 5’ end highlighted by a red asterisk. Radiolabelled template is incubated with KU and DNAPKcs prior to the addition of Shelterin composed of TRF1, TRF2, RAP1, TIN2, POT1 and TPP1. DNase I digested products are then analysed by denaturing Urea-PAGE b-f. DNaseI footprinting of telomere end-binding complexes. Proteins omitted as indicated. Nucleotides from the 5’ telomeric end indicated.
Article Snippet: The
Techniques: Footprinting, Incubation, Binding Assay